TECHNICAL ANNEX

Table 2. First PCR conditions differing by fragment

Fragment A B C D E F
Concentration of primers 0.5 µM 0.5 µM 0.5 µM 0.5 µM 0.5 µM 1.0 µM
Annealing temperature 58ºC 60ºC 54ºC 62ºC 56ºC 52ºC