TECHNICAL ANNEX

Table 1. Primer pairs for first PCR amplification of 6 fragments and for second PCR amplification of 60 segments of the mitochondrial genome

Fragment/segment First PCR Second PCR
L1
Primer
H1
Primer
Length
(bp)
FL2
Primer
H2
Primer
Length
(bp)
Fragment A L77 H3538 3481
1       FL100 H742 680
2       FL398 H1014 654
3       FL700 H1696 1034
4       FL995 H1591 634
5       FL1254 H1828 612
6       FL1485 H2060 613
7       FL1779 H2385 644
8       FL2045 H2669 662
9       FL2332 H3082 788
10       FL2616 H3538 960

 

Fragment/segment First PCR Second PCR
L1
Primer
H1
Primer
Length
(bp)
FL2
Primer
H2
Primer
Length
(bp)
Fragment B L2815 H6158 3358
11       FL2864 H3538 712
12       FL3119 H3876 795
13       FL3455 H4222 805
14       FL3712 H4552 878
15       FL4008 H4552 582
16       FL4250 H4854 642
17       FL4529 H5528 1037
18       FL4827 H5528 739
19       FL5110 H5759 687
20       FL5362 H6009 685

 

Fragment/segment First PCR Second PCR
L1
Primer
H1
Primer
Length
(bp)
FL2
Primer
H2
Primer
Length
(bp)
Fragment C L5545 H9133 3571
21       FL5602 H6158 594
22       FL5890 H6454 602
23       FL6186 H6757 609
24       FL6452 H7005 591
25       FL6716 H7613 935
26       FL6957 H7613 694
27       FL7245 H7758 551
28       FL7497 H8144 685
29       FL7762 H8395 671
30       FL8053 H9133 1118

 

 

Fragment/segment First PCR Second PCR
L1
Primer
H1
Primer
Length
(bp)
FL2
Primer
H2
Primer
Length
(bp)
Fragment D L8281 H11571 3310      
31       FL8345 H9133 826
32       FL8635 H9235 638
33       FL8913 H9483 608
34       FL9213 H10137 962
35       FL9484 H10137 691
36       FL9747 H10629 920
37       FL10028 H10629 639
38       FL10297 H10913 654
39       FL10616 H11179 601
40       FL10910 H11519 647

 

 

Fragment/segment First PCR Second PCR
L1
Primer
H1
Primer
Length
(bp)
FL2
Primer
H2
Primer
Length
(bp)
Fragment E L10796 H14378 3062      
41       FL11183 H11791 646
42       FL11467 H12000 571
43       FL11766 H12451 723
44       FL12057 H12818 799
45       FL12357 H12921 602
46       FL12600 H13492 930
47       FL12889 H13492 641
48       FL13188 H13767 617
49       FL13479 H14102 661
50       FL13721 H14321 638

 

Fragment/segment First PCR Second PCR
L1
Primer
H1
Primer
Length
(bp)
FL2
Primer
H2
Primer
Length
(bp)
Fragment F L13901 H609 3297      
51       FL13986 H14754 806
52       FL14279 H14909 668
53       FL14559 H15162 641
54       FL14837 H15340 541
55       FL15126 H15755 667
56       FL15405 H16016 649
57       FL15696 H81 992
58       FL15948 H81 740
59       FL16221 H342 728
60       FL16504 H581 684